Quantification of a single population in a mixed microbial community using a laser integrated microarray scanner
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
A laser integrated microarray scanner was used to quantify and compare the biomass of Burkholderia cepacia G4 alone, mixed with Afipia sp., and mixed with a trichloroethylene and phenol degrading community. Samples containing B. cepacia G4 were placed in 3-mm diameter wells on gelatin coated glass slides then fixed and immunofluorescently labeled using an IgG conjugate with an attached Alexa TM 546 fluorophore. Linearity and sensitivity of the scanner for biomass quantification were established, and a lower detection limit of 25 mg L 1 (10 3 10 4 cells mL 1 ) was calculated. Growth of B. cepacia G4 alone and in the presence of the TCE degrading community was measured using the scanner. Results suggest that the microarray scanner can be used to quantify the biomass of a single population in a dense mixed microbial community. Key words: biomass, Burkholderia cepacia G4, fluorescent antibody, microarray scanner, mixed community.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it