Myoglobin deficiency in the hearts of phylogenetically diverse temperate-zone fish species
Bibliographic record
Abstract
Previous studies relying upon spectrophotometric methods reported low levels of myoglobin, an intracellular oxygen-binding protein, in oxidative muscles of some sluggish benthic fishes distributed throughout the North Atlantic Ocean. Using immunochemical techniques we show that myoglobin is not expressed in the heart ventricles of Cyclop terus lumpus (Cyclopteridae), Anarhichas lupus (Anarhichadidae), Macrozoarces americanus (Zoarcidae), and Lophius americanus (Lophiidae). Hemitripterus americanus (Hemitripteridae) expresses myoglobin at 2.3 ± 0.2 mg·g wet mass1(mean ± SD). Myoglobin was not detected in oxidative skeletal muscle (pectoral adductor profundus) in either the white-hearted fishes examined or red-hearted H. americanus. Supporting these results, myoglobin messenger RNA was not detected in cardiac muscles of white-hearted fishes by means of either direct Northern blot analysis or by the reverse transcriptase polymerase chain reaction followed by amplification of cDNA product. The partial cDNA sequence of H. americanus myoglobin was determined and shows 86.9% identity with a known teleost myoglobin cDNA from Chionodraco rastrospinosus. The 3' untranslated region of H. americanus is 255 nucleotides longer than the 3' untranslated region of C. rastrospinosus. Comparisons of the deduced amino acid sequence of H. americanus with those of other teleosts show 66.2% sequence identity with Cyprinus carpio, 74.6% with Scomber japonicus, and 80.3% with Thunnus albacares and C. rastrospinosus.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".