Diverting Electron Fluxes to Hydrogen in Mixed Anaerobic Communities Fed with Glucose and Unsaturated C18 Long Chain Fatty Acids
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Bibliographic record
Abstract
Hydrogen (H2) production was maximized and methane (CH4) formation was minimized in a mixed anaerobic culture which was maintained at 21°C and fed glucose plus unsaturated long chain fatty acids (LCFAs). The initial pH in the batch reactors was 7.8±0.2 . The two LCFAs under consideration included linoleic acid (LA) (two CC bonds) and oleic acid (OA) (one CC bond). Hydrogen production was observed when glucose was injected on Day 0 and again after Day 4. The H2 yield in cultures fed LA was less than those receiving OA. The H2 yield reached a maximum of approximately 1.1 mol H2 mol−1 glucose when the LA level was 2,000 mg L−1 . In the case of OA, a maximum yield of 1.3 mol H2 mol−1 glucose was attained with 2,000 mg L−1 . The inhibition caused by the addition of LA or OA diverted a fraction of electrons toward proton reduction. Under maximum H2 production conditions in the LA fed cultures the acetate production pathway was repressed, while in cultures fed OA the acetate pathway was dominant. The amount of CH4 produced decreased with increasing H2 production and the major volatile fatty acids detected were acetate, propionate and butyrate. Small quantities of formate were detected only in cultures fed LA after the first glucose injection. As the LCFA concentration increased, the initial glucose degradation rate decreased.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it