Characterization of a nonphotosynthetic NADP-malic enzyme from germinating soybean cotyledons: interaction with a 94-kDa polypeptide identified as a lipoxygenase
Bibliographic record
Abstract
The major NADP-malic enzyme (NADP-ME) isoform expressed in Glycine max L. cotyledons, leaves, and roots had a subunit molecular mass of 65 kDa and a pI of 6.9. The developmental profile of NADP-ME activity in cotyledons revealed a peak around day 7 post-imbibition (1.53 U·g1fresh mass, 33-fold that of day 1), declining markedly thereafter. NADP-ME was partially purified (33-fold) from 7-d germinated soybean cotyledons with a yield of 41% and a final specific activity of 2.8 U·mg1. NADP-ME had an optimal pH of 7.3 and the Kmvalues for NADP and L-malate were 33 µmol·L1and 0.78 mmol·L1, respectively. The high enzymatic activity found in soybean cotyledons suggests that it plays an important role prior to the establishment of photosynthesis. NADP-ME co-purified with a 94-kDa polypeptide (p94) through various chromatographic steps. Non-denaturing electrophoresis followed by SDS-PAGE and immunoprecipitation assays indicated that NADP-ME is physically associated with p94. Matrix-assisted laser desorption and ionization time of flight MS of p94 tryptic peptides identified p94 as a lipoxygenase. Although the association was verified by different approaches, the functional role for such interaction is still speculative. Possible hypothesis are discussed.Key words: germinating cotyledon, isozyme, lipoxygenase, NADP-malic enzyme, proteinprotein interaction, soybean.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".