Cloning and Characterization of NAD-dependent Deacetylase Sirtuin 2 Homolog from the Silkworm, Bombyx mori.
Bibliographic record
Abstract
Sirtuin2 (Sirt2) is a kind of NAD+-dependent deacetylases ranging from bacteria to human and play an important role inmany biological processes especially in lifespan. We performed genome analysis and protein prediction of Sirt2 of B.mori (BmSirt2). The cDNA sequence of BmSirt2 contains an ORF of 1164 bp encoding 387 amino acid residues with apredicted molecular mass and isoelectric point of 43.37 kDa and 5.02, respectively. This protein shows high degrees ofidentity with other species. Phylogenetic relationship analysis showed that the BmSirt2 protein was in the samesubgroup as the Sirt2 from invertebrate animals. RT-PCR analysis of gene expression in multiple tissues showed thatSirt2 gene was widely expressed in B. mori. BmSirt2 was successfully expressed in E. coli with a molecular mass of48.0 kDa. The identification of the recombinant protein by MALDI-TOF-MS and western blotting showed this fusionprotein was the correct one.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".