An Optimized Relaxation-Based Coherence Transfer NMR Experiment for the Measurement of Side-Chain Order in Methyl-Protonated, Highly Deuterated Proteins
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Bibliographic record
Abstract
Relaxation violated coherence transfer NMR spectroscopy has emerged as a powerful experimental tool for the quantitative measurement of amplitudes of motion of methyl containing side-chains. Typically, the experiments, performed on proteins that are highly deuterated and methyl-protonated, monitor the build-up of methyl (1)H double-quantum magnetization. Because all three protons in a methyl group are degenerate, such coherences can only result from differential relaxation of transverse magnetization components, which in turn reflect the extent and time-scale of motion of the methyl probe [Tugarinov, V., Sprangers, R.; Kay, L.E. J. Am. Chem. Soc. 2007, 129, 1743-1750]. We show here that a 50% gain in the sensitivity of the experiment can be achieved through selection of (1)H triple-quantum coherence, thereby significantly increasing the utility of the approach. A theoretical treatment rationalizes the sensitivity gain that is subsequently verified through experiment. The utility of the methodology is demonstrated on a number of proteins, including the 360 kDa α(7)α(7) "half-proteasome".
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Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
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