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Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)

2004· article· en· W1980521044 on OpenAlexfundno aff
André Freire Cruz

Bibliographic record

VenueActa Botanica Brasilica · 2004
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicMycorrhizal Fungi and Plant Interactions
Canadian institutionsnot available
FundersAgriculture and Agri-Food CanadaAlexander von Humboldt-Stiftung
KeywordsSporeElectron energy loss spectroscopyTransmission electron microscopyLiquid nitrogenFungusVacuoleBacteriaBotanyUltrastructureNitrogenBiologyChemistryMaterials scienceBiochemistryNanotechnologyPaleontologyOrganic chemistry

Abstract

fetched live from OpenAlex

The storage structures of spores of Gigaspora margarita Becker & Hall, an arbuscular mycorrhizal fungus, were analyzed by a transmission electronic microscope (TEM) and electron energy loss spectroscopy (EELS). The spores were hydrated, cryofixed using high pressure freezing, submitted to freeze substitution, embedded and prepared for TEM observations. The cryotechniques revealed that the storage structures in the spores are composed of lipids, electron dense granules (EDG), protein bodies and glycogen particles. The EELS detected the presence of nitrogen (N) and carbon (C) in these storage structures and in Bacteria-like organisms (BLO). Some amount of P was detected in the vacuoles and in BLO. The TEM and EELS techniques indicate the presence of storage structures in the fungal spore, and some essential elements (N, P and C) in these structures.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.001

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.218
Teacher spread0.209 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations13
Published2004
Admission routes1
Has abstractyes

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Same venueActa Botanica BrasilicaSame topicMycorrhizal Fungi and Plant InteractionsFrench-language works237,207