Duplication of<i>cyb-3</i>(cyclin B3) suppresses sterility in the absence of<i>mdf-1/MAD1</i>spindle assembly checkpoint component in<i>Caenorhabditis elegans</i>
Bibliographic record
Abstract
mdf-1/MAD1 is a conserved spindle assembly checkpoint component that is essential for the survival of Caenorhabditis elegans. Previously, using a dog-1(gk10)/FANCJ mutator strain, we have isolated a suppressor of mdf-1(gk2) sterility. This suppressor, named such-4, was demonstrated to be a tandem duplication that contained 62 putative protein coding genes. We apply here the recently developed Mos1-mediated single-copy insertion (MosSCI) method to study this copy number variation (CNV) in C. elegans and show that such-4 is caused by the duplication of a single gene cyb-3, illustrating the power of MosSCI-mediated single-gene duplications for uncovering gene dosage genetic interactions. Importantly, we show here, for the first time, that doubling the CYB-3 (Cyclin B3) dosage suppresses sterility in the absence of the essential spindle assembly checkpoint component MDF-1 without causing a delay in the onset of anaphase.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".