A note on four nonradioactive labeling systems for dot hybridization detection of potato viruses
Bibliographic record
Abstract
Complementary DNA clones of genomic RNAs of potato ( Solarium tuberosum ) viruses S (PVS), X (PVX) and Y (PVY) were produced and tested for their capacity to hybridize with various plant virus RNAs. PVS clone S12 and PVX clone X6 were found to be very specifie to PVS and PVX RNA respectively, whereas PVY clone Y10 strongly hybridized with PVY RNA and weakly with PVS RNA. Four commercial, nonradioactive Systems of nucleic acid labeling and detection were compared to the usual 32 P-labeled probe using dot hybridization experiments. Colorimetric detection of digoxigenin-labeled DNA probes gave a level of sensitivity of 1 ng of virions (60 pg of RNA), similar to autoradiography of 32 P-labeled probes. Sulfonated, biotinylated and peroxidase-labeled probes were slightly less sensitive, allowing detection of 600 pg of viral RNA.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".