A Generalized Tip-Membrane Contact Detection Algorithm for Automated Single Cell Electroporation Using Statistical Process Control
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Bibliographic record
Abstract
This work presents a fully automated method for detecting the contact between a microcapillary tip and a cell membrane based on a statistical process control (SPC) algorithm known as the double-sided cumulative sum (or "cusum"). By analyzing current measurements obtained through a microcapillary electrode, the proposed goal of this system is to determine when tip-to-membrane (tip-membrane) contact occurs using thin adhered cells (e.g., less than 10 μm) for the purposes of fully automated robotic-assisted, single cell electroporation (SCE) - a powerful method of gene transfection. This SPC algorithm is robust against uncontrollable system parameters such as system noise common in electrode-based systems, nonstationary processes, and variations in the physical parameters of cells. The proposed algorithm was successfully demonstrated on adhered mammal cells as small as 4 μm in thickness and using tip-placement velocities from 1 to 8 μm/s. In addition, a novel method of experimentation is described correlating optical measurements between tip-membrane proximity and changes in i <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">cct</sub> during the tip-placement sequence.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it