Cloning, Expression and Characterization of PnLOX2 Gene Related to Aspergillus flavus-resistance from Peanut (Arachis hypogaea L.) Seed Coat
Why this work is in the frame
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Bibliographic record
Abstract
Aspergillus flavus is one of the major diseases of peanut. The objective of the study was to clone PnLOX2 gene from peanut seed coat and analyze its expression. Real-time RT-PCR analysis indicated that the gene was overexpressed in the challenge of Aspergillus flavus and the greatest expression occurred 10 days after inoculation. The full length of PnLOX2 gene was 2592bp and encoded a 97.5kDa protein containing 863 amino acids. The fusion protein (apparent 121.5kDa) only existed in the precipitation and the maximum was obtained by inducing at 37ºC for 3.5h. As a result of response to the challenge of Aspergillus flavus, PnLOX2 gene was more greatly expressed in the resistant genotype than in the susceptible genotype. It indicated that PnLOX2 gene was closely related to Aspergillus flavus-resistance of peanut. Further investigations were needed to bacteriostatically identify the gene in vitro.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it