Quantitative Determination of Chemical Constituents from Seeds of Nigella sativa L. by using HPLC-UV and Identification by LC-ESI-TOF
Bibliographic record
Abstract
Nigella sativa Linn. belongs to the Ranunculaceae family and is an indigenous herbaceous plant that is more commonly known as the fennel flower plant. The plant is also known as black cumin (English) and black-caraway (USA). The spicy seeds from this plant have medicinal usage dating back to the ancient Egyptians, Greeks and Romans. In Egypt and the Middle East the black seed oil is popularly used for certain cases of chronic cough and bronchial asthma [1,2]. An HPLC method was developed for the simultaneous determination of nine compounds of Nigella sativa L. The separation was achieved within 23 minutes by using C-18 column material, a water/acetonitrile mobile phase, both containing 0.1% acetic acid gradient system and a temperature of 35°C. The method was validated for linearity, repeatability, limits of detection (LOD) and limits of quantification (LOQ). The LOD and LOQ of nine compounds were found to be in the range from 0.09–10 µg/mL and 0.3–25 µg/mL, respectively. The wavelength used for quantification with the diode array detector was 205 and 260 nm. The seeds of N. sativa and commercial products showed the presence of all nine compounds. LC-mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification of compounds in Nigella sativa L. samples. This method involved the use of [M+H] + and [M+Na] + ions in the positive ion mode with extractive ion monitoring (EIM). Acknowledgements: This research is funded in part by “Science Based Authentication of Dietary Supplements” Funded by the Food and Drug Administration grant number 2 U01 FD 002071-07. References: [1] Zawahry MR, (1963), Kongr Pharm Wiss Vortr Origenatitt, 23: 193–203. [2] Khan A, (1999), Inflammopharmacology, 7(1): 15–35. Fig. 1 HPLC chromatograms of standard mix, plant sample and dietary supplement at 260 nm (1) magnoflorine, (2) Kaempferol-3- O -β-glucopyranosyl (1 → 2)- O -β-galactopyranosyl (1 → 2)- O -glucopyranoside, (3) sieboldianoside A, (4) tauroside H2, (5) tauroside G3, (6) decaisoside D, (7) sapindoside B, (8) thymoquinone, (9) tauroside E.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".