Nisin: A Novel Substrate for Glutathione S‐Transferase Isolated from Fresh Beef
Bibliographic record
Abstract
ABSTRACT: Little success has been reported on the use of the antimicrobial peptide nisin in meat products. Previous research in our laboratory hypothesized that nisin is inactivated in raw meat systems by an enzymatic reaction with glutathione. We isolated glutathione S‐transferase from fresh beef muscle and examined the in vitro enzymatic reaction between nisin and glutathione. The products of the reaction were analyzed by antibacterial‐activity assays and MALDI‐TOF MS. Under optimum reaction temperatures, the results showed that 3 glutathione molecules could conjugate to 1 nisin molecule, suggesting that multiple dehydro residues are involved. Assays for antibacterial activity confirmed that all activity was lost. Results support the inactivation of nisin by glutathione.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".