A hyaluronidase from the Snake Venom of Agkistrodon Blomhoffii Ussurensis of Changbai Mountain: Isolation and Characterization
Bibliographic record
Abstract
Snake venom is a complex mixture of protein and peptide toxins. Hyaluronidase a‘spreading factor’ has not beenstudied extensively in snake venom. In this paper, we describe the purification and characterization of ahyaluronidase from the snake venom of Agkistrodon blomhoffii ussurensis of Changbaimountain. Hyaluronidasehas been purified by the successive chromatography on a DEAE-Sephadex A-25, on CM- Sephadex C-25 and onSephadex G-75 columns on the basis of the purification method of thrombin-like enzyme (TLE). The molecularmass was found to be 35.0 kDa by SDS-polyacrylamide gel electrophoresis. Hyaluronidase was 5.66 percent ofthe snake venom of Agkistrodon blomhoffii ussurensis of Changbai mountain, the activity of mass (u/g) was437218.5. The pH optimum for the enzyme was in the rang of 3.0-4.0 and 6.0-6.5,maximum activity wasobtained at 37?, and the optimum concentration of Na+(mol/L) was in the rang of 0.3-3.0, the Km forhyaluronidase, using hyaluronic acid as substrate, was 4.633 × 10-3 mg/ml. The hyaluronidase had not theinhibition effect to growth of the Hela cell and HepG-2 cell in vitro through the experiment.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".