175 FOLLICULAR DIAMETER AND OVULATION RATE IN NELLORE COWS TREATED WITH LH
Bibliographic record
Abstract
The aim of the present experiment was to verify the correlation between follicular diameter and ovulation rate in Nellore cows submitted to LH treatment to add information to the limited literature about this subject. A preliminary test (experiment 1) was performed to determine the lowest dose of LH necessary to induce ovulation in follicles with diameter =10 mm. At a random stage of the estrous cycle (D0), Nellore cows (n = 20) received a progesterone intravaginal device (1.0 g, Primer®, Tecnopec, Sao Paulo, Brazil) and 2.5 mg of estradiol benzoate (EB, i.m., Estrogin®; Farmavet, Sao Paulo, Brazil). Eight days later (D8) PGF2a was administered (150 µg of D-cloprostenol, i.m., Prolise® ARSA S.R.L., Buenos Aires, Argentina) and the device was removed. Twenty-four hours after device removal, cows were treated with EB (1.0 mg, i.m.) and 48 h afterward; ovulation was determined by ultrasonography (US, Aloka 900, Tokyo, Japan). Three days after ovulation, the follicular growth was daily observed by US, and when the follicles reached =10 mm diameter, the cows were randomly allocated in 4 groups and treated with different doses of LH (i.m., Lutropin-V®, Bioniche, Ontario, Canada): Groups 1.56; 3.12; 6.25, and 12.5 mg. The data were analyzed by Fisher’s Exact Test (PROC FREQ, SAS). The ovulation rates were 0% (0/5), 80% (4/5), 80% (4/5), and 80% (4/5) respectively, and it was lower in group 1.56 when compared with the others (P < 0.02). Taking in account the results above, the dose of 6.25 mg to induce ovulation in Nellore cows (n = 58, experiment 2) submitted to the same protocol used in experiment 1 and randomly allocated in 5 groups according to follicular diameter at the time of LH administration: G1 (7.0–7.5 mm), G2 (7.6–8.0 mm), G3 (8.1–8.5 mm), G4 (8.6–9.0 mm), and G5 (9.1–10.0 mm). The results were analyzed by Logistic Regression (PROC GENMOD, SAS). The ovulation rates were 0% (0/11), 20% (2/10), 36% (4/11), 36% (4/11), and 90% (9/10), respectively, for G1, G2, G3, G4, and G5. When the data from 2 groups (except G5) were combined, G1 + G2 (7.0–8.0 mm), G3 + G4 (8.1–9.0 mm), and G5 (9.1–10.0) ovulation rates were, respectively, 9% (2/21), 36% (8/22), and 90% (9/10). There were significant differences when comparing G1 + G2 v. G5 (P < 0.01), G3 + G4 v. G5 (P < 0.02) and G1 + G2 v. G3 + G4 (P < 0.03). After LH application, the smallest follicle that ovulated had a diameter of 7.65 mm. In summary, although LH administration induced ovulation in one follicle of 7.65 mm, high ovulation rates were observed only when the follicles reached <9.0 mm. These data will be used to study the correlation between ovulation rate and expression of isoforms of LH mRNA receptors in theca and granulosa cells. Fellowship from *FAPESP (Sao Paulo, Brazil) and **CAPES (Brasilia, Brazil).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".