An Anti-Cancer Cordycepin Produced by Cordyceps militaris Growing on the Dead Larva of Bombyx mori Silkworm
Bibliographic record
Abstract
In this study, we introduced the dead pupa and larva of Bombyx mori silkworms as nutrition medium for the growing of different fungi including Cordyceps militaris, Isaria tenuipes and Isaria farinose to produce Cordyceps mycelia and anti-cancer cordycepin. The anti-proliferative and anti-migratory activities of cordycepin extracted toward cancer cells were investigated. We found that the dead silk larva which contained high carbohydrate and moisture but low fat content could be a good host for the growth of Cordyceps militaris and Isaria tenuipes to produce Cordyceps. The cordycepin extracted from Cordyceps mycelia of Cordyceps militaris grown on dead silk larva showed the highest anti-proliferative potential toward human non-small cell lung cancer NCI-H460 cells with a half maximal inhibitory concentration (IC50) of 0.7 µM. Furthermore, the viability of human lung adenocarcinoma epithelial A549 cell line remained < 20% while that of human airway epithelial Calu-3 cell line was < 40% after treated with the cordycepin extracted (0.125-2 µM) due to the disruption of cell membrane by cordycepin. We also found that our cordycepin (0.25-2 µM) could inhibit the migration of A549 cells. On the other hand, the cordycepin was not toxic to small airway epithelial cells (SAEC) of non-cancer cells. Therefore, Cordyceps militaris grown on the dead larva of B. mori silkworms was introduced as a promising source for the production of Cordyceps mycelia and anti-cancer cordycepin without toxicity to non-cancer cells.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".