Expression of borage Δ6 desaturase in <i>Saccharomyces cerevisiae</i> and oilseed crops
Bibliographic record
Abstract
The borage Δ6 desaturase is a modular protein that contains a cytochrome b 5 -like domain attached to the N-terminus of the main catalytic domain. When the full-length open reading frame was expressed in two types of Saccharomyces cerevisiae strains, the endogenous cytochrome b 5 -disrupted strain and the cytochrome b 5 -competent wild type strain, the expressed enzyme introduced a double bond at the Δ6 position into the endogenous substrates 16:1(9) and 18:1(9), and also into the exogenously fed fatty acid substrates 18:2(9,12) and 18:3(9,12,15). However, the preferred substrates of the enzyme when expressed in yeast were 18:2(9,12) and 18:3(9,12,15), whereas 16:1(9) and 18:1(9) were relatively poor substrates. When the partial desaturase sequence with a deletion of the cytochrome b 5 -like domain at the N-terminus was introduced into the yeast strains, the truncated enzyme could not desaturate any of the supplied substrates. Introduction of the full-length cDNA into flax (Linum usitatissimum L.) under the control of a constitutive (35S) promoter resulted in accumulation of the two Δ6 desaturated fatty acids (18:3(6,9,12) and 18:4(6,9,12,15)), in one elite line, forming up to 22% of the total fatty acids in the stem, 19% in the root, and 11% in the leaf. Introduction of the desaturase into Brassica juncea (L.) Czern. under the control of the Brassica napus L. napin promoter resulted in accumulation of the Δ6 desaturated fatty acids at levels of up to 13% of the total fatty acids in mature seeds.Key words: borage, Δ6 desaturase, γ-linolenic acid, stearidonic acid, Brassica juncea, flax.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".