Demonstration of the Deposition and Modification of Dietary Fatty Acids in Pinniped Blubber Using Radiolabelled Precursors
Why this work is in the frame
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Bibliographic record
Abstract
Radioisotopes are commonly used to study the in vivo metabolism and deposition of dietary fatty acids in adipose tissue. The application of this approach to pinnipeds is problematic because of their large mass and blubber fat content. We have developed a method where labelled lipids can be fed to seals at financially feasible levels, with the radioactivity in individual fatty acids isolated from blubber detected with standard laboratory equipment. A combination of techniques including argentation thin layer chromatography, high performance liquid chromatography with ultraviolet detection, and independent liquid scintillation counting were employed. Juvenile gray seals (Halichoerus grypus) were fed either 0.5 mCi 3H‐labelled triolein (18:1n‐9, n = 2) or palmitic acid (16:0, n = 2). Blubber samples were taken 12 h later, and the radioactivity in individual fatty acids was determined. Radioactivity was detected in only 18:1 from the animals fed 3H‐labelled triolein, indicating direct deposition without modification. Both animals fed 3H‐labelled palmitic acid showed clear peaks of radioactivity in 16:0; however, there was also significant activity (23%–29%) found in the desaturation product 16:1. Our results demonstrate that this method is sufficiently sensitive to track the deposition of labelled dietary lipids as well as modification products of ingested fatty acids and will be important in the application of fatty acid signatures to study predator diets.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it