Additional evidence of the facultative intracellular nature of the fish bacterial pathogen Piscirickettsia salmonis
Bibliographic record
Abstract
Piscirickettsia salmonis es un microorganismo altamente contagioso y virulento que afecta a la salmonicultura mundial desde el ltimo tercio del siglo pasado y del cual sus mecanismos de sobrevivencia intracelular son completamente desconocidos. Despus de algunos reportes recientes en donde se cuestiona su condicin de intracelular obligado, hemos decidido mostrar evidencia adicional para cambiar este paradigma, llevando a cabo experimentos tanto clsicos como moleculares que confirman su naturaleza de intracelular facultativo. En este reporte se demuestra inequvocamente que la bacteria recuperada desde cultivos celulares infectados, de placas de agar o de medio lquido, es el mismo organismo, el cual cumple con los postulados de Koch. Adems, anlisis genticos y protemicos confirman que la bacteria obtenida de diferentes fuentes de crecimiento corresponde a la misma cepa tipo LF-89, la que fue originalmente descrita por Fryer en 1992. Sin embargo, el crecimiento de la bacteria, tanto en medios libres de clulas como en cultivo celular, es subptimo, por lo cual se requieren ms anlisis para entender completamente la biologa del patgeno. Interesantemente, en este trabajo se logr la mantencin de la bacteria en medio lquido, pero a una tasa muy baja de crecimiento. En conclusin, y sumado a reportes anteriores, hemos confirmado la naturaleza intracelular facultativa de este patgeno de peces de cultivo.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.006 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".