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Record W1994509491 · doi:10.1107/s174430911100515x

Structure of<i>Francisella tularensis</i>peptidyl-tRNA hydrolase

2011· article· en· W1994509491 on OpenAlexaff
Teresa E. Clarke, V. Romanov, Robert Lam, Scott A. Gothe, Srinivasa R. Peddi, E. Razumova, Richard S. A. Lipman, Arthur Branstrom, Nickolay Y. Chirgadze

Bibliographic record

VenueActa Crystallographica Section F Structural Biology and Crystallization Communications · 2011
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBacillus and Francisella bacterial research
Canadian institutionsUniversity of TorontoCanada Research ChairsUniversity Health Network
Fundersnot available
KeywordsFrancisella tularensisHydrolaseTransfer RNATularemiaEnzymeBiologyBiochemistryRifamycinBacteriaFrancisellaCloning (programming)Active siteChemistryMicrobiologyAntibioticsGeneticsRNAGeneVirulence

Abstract

fetched live from OpenAlex

The rational design of novel antibiotics for bacteria involves the identification of inhibitors for enzymes involved in essential biochemical pathways in cells. In this study, the cloning, expression, purification, crystallization and structure of the enzyme peptidyl-tRNA hydrolase from Francisella tularensis, the causative agent of tularemia, was performed. The structure of F. tularensis peptidyl-tRNA hydrolase is comparable to those of other bacterial peptidyl-tRNA hydrolases, with most residues in the active site conserved amongst the family. The resultant reagents, structural data and analyses provide essential information for the structure-based design of novel inhibitors for this class of proteins.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.675
Threshold uncertainty score0.874

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.262
Teacher spread0.244 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations23
Published2011
Admission routes1
Has abstractyes

Explore more

Same venueActa Crystallographica Section F Structural Biology and Crystallization CommunicationsSame topicBacillus and Francisella bacterial researchFrench-language works237,207