LF 15-0195 Generates Synergistic Tolerance by Promoting Formation of CD4+CD25+CTLA4+ T Cells
Bibliographic record
Abstract
The purpose of the present study was to investigate the influence of 15-deoxyspergualin (LF) on the phenotypes and functions of dendritic cells (DCs) and T cells and to further illustrate the mechanism of LF-inducing immunologic tolerance. DCs from mice were cultured and treated with varying doses of LF at specific time-points. Fluorescence-activated cell sorting (FACS) was used to verify the changes of phenotypes in the cultured DCs labeled with fluorescent antibody. DCs were also used as stimulators in mixed leukocyte reaction to detect their ability to stimulate T-cell proliferation. DCs and T cells, treated with or without LF, were cultured together; phenotypes and cytokine profile of the T cells were identified and assayed by FACS and enzyme-linked immunosorbent assay. LF induced a dose- and time-dependent suppression of maturation of DCs and a dose-dependent suppression of T-cell proliferation in mixed leukocyte reaction when LF-treated DCs were used as stimulators. LF-treated DCs, cultured with naive T cells, could promote the formation of CD4+CD25+CTLA4+ T-cell subtypes and the production of higher levels of interleukin-10. It was suggested that the mechanism of LF-induced tolerance was inhibiting maturation and function of DC and inducing the formation of regulatory T-cell subtype by "suppressor DCs" to achieve a new immune balance.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".