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Record W1996589341 · doi:10.1126/stke.2003.170.pl5

Measuring Chemotaxis and Chemokinesis: The Under-Agarose Cell Migration Assay

2003· article· en· W1996589341 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueScience s STKE · 2003
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCellular Mechanics and Interactions
Canadian institutionsUniversity of Calgary
Fundersnot available
KeywordsChemotaxisChemokinesisMulticellular organismCell biologyChemotaxis assayCytoskeletonBiologyCell migrationCell signalingCell polaritySignal transductionCellChemistryReceptorBiochemistry

Abstract

fetched live from OpenAlex

Chemotaxis is the primary mechanism by which cell movements are directed within multicellular organisms, and it is a major component of embryonic development, wound healing, and immune responses. Chemotaxis involves a complex cascade of events--formation of signaling complexes, receptor polarization, adhesion molecule activation, and cytoskeletal reorganization. Previous assay methods were limited in several ways that reduced users' abilities to obtain quantitative data or to control conditions precisely. We describe a unique chemotactic assay that can incorporate multiple chemotactic gradients in different spatial and temporal combinations. In addition, this assay is easily adapted for live-cell imaging and fluorescent microscopy. With its relative simplicity, flexibility, and precision, this method is a key tool for the study of cellular chemotactic responses and the signaling processes underlying them.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.030
Threshold uncertainty score0.233

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.223
Teacher spread0.207 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it