Induction of callus formation from difficile date palm protoplasts by means of nurse culture
Why this work is in the frame
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Bibliographic record
Abstract
We report here for the first time callus formation from protoplasts in date palm (Phoenix dactylifera L.). Protoplasts were isolated from young leaves of offshoots and embryogenic calli in Deglet nour and Takerboucht genotypes. The protoplast yield depended on genotype, donor plant material, mixture of enzyme solution, and incubation time. With regard to the donor material, the best response was obtained with callus. Cell division was induced in both liquid culture and nurse culture. The best donor material for cell division was callus and the best response was obtained with the feeder layer, which induced a division rate of 30% in Deglet nour and 15% in Takerboucht genotypes. The dividing cells developed to microcalli on the feeder layer; the microcalli developed to calli on modified MS medium; however, the calli failed to regenerate into roots or shoots.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it