The Kinesin Motor Protein Cut7 Regulates Biogenesis and Function of Ago1‐Complexes
Bibliographic record
Abstract
Argonaute proteins are the effectors of small RNA-dependent gene-silencing pathways. In the cytoplasm, they are incorporated into large mobile ribonucleoprotein (RNP) complexes that travel along microtubules. We used a genetic screen to identify the microtubule-associated motor that interacts with Ago1-containing RNPs. Here, we report that activity of the kinesin family member Cut7 is important for biogenesis and/or stability of Ago1-containing RNPs in the cytoplasm. Results from pulldown and coimmunoprecipitation assays indicate that Cut7 interacts with Ago1 as well as its two cognate binding proteins, Dcr1 and Rdp1. Loss of Cut7 activity was associated with increased levels of reverse centromeric transcripts, presumably because of a defect in post-transcriptional gene silencing. Overexpression of the Ago1-binding region of Cut7 resulted in loss of microscopic Ago1-containing RNPs. Together, these results suggest that microtubule motor proteins function in the biogenesis and function of gene-silencing machinery in the cytoplasm.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".