Molecular forms and thermal and kinetic properties of purified glutathione reductase from two populations of barnyard grass (<i>Echinochloa crus-galli</i> (L.) Beauv.: Poaceae) from contrasting climatic regions in North America
Bibliographic record
Abstract
The thermal, kinetic, and electrophoretic properties of purified glutathione reductase (GR; EC 1.6.4.2) were analyzed in plants from two ecotypes of barnyard grass (Echinochloa crus-galli (L.) Beauv.: Poaceae) originating from sites of contrasting climates in Quebec (QUE) and Mississippi (MISS). Crude and purified GR preparations from plants of both ecotypes consisted of one homodimer isomorph with the same electrophoretic mobility in polyacrylamide gels, a similar molecular mass for the native enzyme (98 kDa) and for each subunit of the dimer (44 kDa), and an identical pI of 5.9. The electrophoretic profile of GR purified from cold-acclimated plants at 14°C light (L) : 8°C dark (D) for 10 days was similar to that of GR from plants grown at 26°C L : 20°C D. Specific activities of purified GR from QUE plants were significantly higher than those of MISS plants. In vitro GR activities from QUE and MISS plants were not differentially affected by thermodenaturation at 55 or 65°C or by cold treatments at 2°C. A...
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".