Axially illuminated fluorescence imaging detection for capillary isoelectric focusing on Teflon capillary
Bibliographic record
Abstract
An axially illuminated laser induced fluorescence (LIF) whole column imaging detection (WCID) method was developed for capillary isoelectric focusing (CIEF). In this method, an argon ion laser was used as the excitation source and the isoelectric focusing process was monitored dynamically by a thermoelectrically (TE) cooled (charge coupled device) CCD camera. Low refractive index PTFE capillary was used in the experiment, and electroosmotic flow (EOF) and protein adsorption were minimized by dynamic conditioning of the capillary with methyl cellulose (MC). With the addition of a small amount of high refractive index organic solvent such as glycerol in the sample mixture, the laser beam was axially propagated with total internal reflection. Stray light and scattering light originating from the wall of the capillary were minimized. The instrumentation is simple and the method is sensitive. Two naturally fluorescent proteins (R-phycoerythrin and green fluorescent protein) were separated, and a concentration detection limit (LOD) of 10−13 M or mass LOD of 10−19 mol for R-phycoerythrin was obtained.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".