Stability of cyclopiazonic acid in solution
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Cyclopiazonic acid (CPA) is an important mycotoxin given its toxicity and prevalence in foods and feeds. There is tremendous interest in developing analytical methods that include CPA as part of a multi-residue mycotoxin routine, but there appears to be considerable difficulty in analysing it using liquid chromatography with electrospray ionisation tandem mass spectrometry (LC-MS/MS). During the development of a multi-residue method for mycotoxins including CPA, a number of issues were discovered under routine and common analytical conditions that have an impact on the determination of CPA, including: (1) at the ng/ml level CPA reacts with ambient oxygen from the headspace of the vial, an effect that decreases its concentration linearly; (2) CPA readily adsorbs to plastic in a reversible fashion; (3) CPA is acid hydrolysed with formic acid; (4) CPA reacts with the column stationary phase affecting chromatographic parameters; and (5) CPA presents significant carry-over issues. In an effort to find solutions to these problems we found that CPA can be protected from reacting with oxygen by adding 1 µg/ml ascorbic acid and that its carry-over can be reduced to a negligible level by injecting ammonia between injections of solutions containing CPA, even with formic acid in the mobile phase. Chromatographic conditions for CPA have been optimised in consideration of all of the aforementioned concerns.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it