Transgene Expression under the Adenoviral Major Late Promoter, Tripartite Leader Sequence and E1 Genes in Absence and Presence of Adenovirus Infection
Bibliographic record
Abstract
Active gene expression driven by the adenoviral major late promoter (MLP) and the tripartite leader sequence (TPL) influenced their utilization to drive vaccines expression. It is unclear if the complete TPL sequence is required for this active expression or if it can be further boosted in presence of the adenoviral E1 genes. In this study, we investigated the effect of TPL exones and E1 genes, out of the viral genome or under adenoviral infection, on green fluorescence protein (GFP) expression driven by the MLP. Gradual increase in the transcript copies with the addition of TPL exons indicated an additive effect especially under infection. The presence of E1 genes significantly enhanced transgene transcription. Infected cells showed higher GFP transcription and translation than non-infected cells. These results indicate higher exogenous gene expression driven by the MLP can be achieved in the presence of adenoviral components other than the TPL and E1 genes.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".