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Record W2002294758 · doi:10.1016/j.jim.2009.12.007

Protein quantification by chemiluminescent Western blotting: Elimination of the antibody factor by dilution series and calibration curve

2009· article· en· W2002294758 on OpenAlexafffund
Steve J. Charette, Herman Lambert, Philippe J. Nadeau, Jacques Landry

Bibliographic record

VenueJournal of Immunological Methods · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
Topicbioluminescence and chemiluminescence research
Canadian institutionsHôtel-Dieu de QuébecInstitut Universitaire de Cardiologie et de Pneumologie de QuébecUniversité Laval
FundersCanadian Institutes of Health ResearchCanada Research Chairs
KeywordsBlotWestern blotContext (archaeology)ChemiluminescenceCalibration curveChemistryCalibrationAntibodyDilutionQuantitative analysis (chemistry)ChromatographyMolecular biologyBiologyBiochemistryMathematicsImmunologyDetection limitStatisticsPhysics

Abstract

fetched live from OpenAlex
No abstract in any covered source. Its absence is recorded, not treated as a negative.

No abstract. This is not a gap in this database; OpenAlex has none either. 23.3% of the frame is in this state, and the screen finds HALF as much metaresearch here, so the absence is a measured bias rather than a missing field.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.012
metaresearch head score (Gemma)0.009
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.020
Threshold uncertainty score0.066

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0120.009
Meta-epidemiology (narrow)0.0120.007
Meta-epidemiology (broad)0.0060.004
Bibliometrics0.0080.008
Science and technology studies0.0030.004
Scholarly communication0.0030.006
Open science0.0080.003
Research integrity0.0040.014
Insufficient payload (model declined to judge)0.0200.017

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.360
Teacher spread0.337 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations42
Published2009
Admission routes2
Has abstractno

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