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Naturally transmitted segmented filamentous bacteria segregate with diabetes protection in nonobese diabetic mice

2011· article· en· 424 citations· W2002553376 on OpenAlex· 10.1073/pnas.1108924108

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Canadian funderA Canadian agency funded it. The work may carry no Canadian affiliation at all.

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Opus teacher head0.023
GPT teacher head0.249
Teacher spread
0.226 · how far apart the two teachers sit on this one work
Validation status
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Abstract

Vertebrates typically harbor a rich gastrointestinal microbiota, which has coevolved with the host over millennia and is essential for several host physiological functions, in particular maturation of the immune system. Recent studies have highlighted the importance of a single bacterial species, segmented filamentous bacteria (SFB), in inducing a robust T-helper cell type 17 (Th17) population in the small-intestinal lamina propria (SI-LP) of the mouse gut. Consequently, SFB can promote IL-17-dependent immune and autoimmune responses, gut-associated as well as systemic, including inflammatory arthritis and experimental autoimmune encephalomyelitis. Here, we exploit the incomplete penetrance of SFB colonization of NOD mice in our animal facility to explore its impact on the incidence and course of type 1 diabetes in this prototypical, spontaneous model. There was a strong cosegregation of SFB positivity and diabetes protection in females, but not in males, which remained relatively disease-free regardless of the SFB status. In contrast, insulitis did not depend on SFB colonization. SFB-positive, but not SFB-negative, females had a substantial population of Th17 cells in the SI-LP, which was the only significant, repeatable difference in the examined T-cell compartments of the gut, pancreas, or systemic lymphoid tissues. Th17-signature transcripts dominated the very limited SFB-induced molecular changes detected in SI-LP CD4(+) T cells. Thus, a single bacterium, and the gut immune system alterations associated with it, can either promote or protect from autoimmunity in predisposed mouse models, probably reflecting their variable dependence on different Th subsets.

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The record

Venue
Proceedings of the National Academy of Sciences
Topic
Gut microbiota and health
Field
Biochemistry, Genetics and Molecular Biology
Canadian institutions
Funders
National Institute of Diabetes and Digestive and Kidney DiseasesNational Institute of Allergy and Infectious DiseasesCanadian Institutes of Health Research
Keywords
BiologySegmented filamentous bacteriaInsulitisImmune systemAutoimmunityImmunologyPopulationNOD miceAutoimmune diseaseNodGut floraLamina propriaGastrointestinal tractDiabetes mellitusAntibodyGeneticsMedicineEndocrinology
Has abstract in OpenAlex
yes