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Record W2003870059 · doi:10.1101/gr.152942.112

<i>CCAT2</i>, a novel noncoding RNA mapping to 8q24, underlies metastatic progression and chromosomal instability in colon cancer

2013· article· en· W2003870059 on OpenAlexaff
Hui Ling, Riccardo Spizzo, Yaser Atlasi, Milena S. Nicoloso, Masayoshi Shimizu, Roxana S. Redis, Naohiro Nishida, Roberta Gafà, Jian H. Song, Zhiyi Guo, Cristina Ivan, Elisa Barbarotto, Ingrid de Vries, Xinna Zhang, Manuela Ferracin, Mike Churchman, Janneke F. van Galen, Berna Beverloo, Maryam Shariati, Franziska Haderk, Marcos R. Estecio, Guillermo Garcia‐Manero, Gijs A. Patijn, D. C. Gotley, Vikas Bhardwaj, Imad Shureiqi, Subrata Sen, Asha S. Multani, James Welsh, Ken Yamamoto, Itsuki Taniguchi, Min‐Ae Song, Steven Gallinger, Graham Casey, Stephen N. Thibodeau, Loı̈c Le Marchand, Maarit Tiirikainen, Sendurai A. Mani, Wei Zhang, Ramana V. Davuluri, Koshi Mimori, Masaki Mori, Anieta M. Sieuwerts, John W.M. Martens, Ian Tomlinson, Massimo Negrini, Ioana Berindan‐Neagoe, John A. Foekens, Stanley R. Hamilton, Giovanni Lanza, Scott Kopetz, Riccardo Fodde, George A. Călin

Bibliographic record

VenueGenome Research · 2013
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCancer-related molecular mechanisms research
Canadian institutionsLunenfeld-Tanenbaum Research InstituteMount Sinai Hospital
FundersNational Cancer InstituteUniversity of Texas MD Anderson Cancer CenterNederlandse Organisatie voor Wetenschappelijk OnderzoekCancer Research UKWellcome Trust
KeywordsBiologyWnt signaling pathwayLong non-coding RNAGeneticsColorectal cancerTCF7L2SNPChromosome instabilityCancerSingle-nucleotide polymorphismCancer researchGeneRNAGenotype

Abstract

fetched live from OpenAlex

The functional roles of SNPs within the 8q24 gene desert in the cancer phenotype are not yet well understood. Here, we report that CCAT2, a novel long noncoding RNA transcript (lncRNA) encompassing the rs6983267 SNP, is highly overexpressed in microsatellite-stable colorectal cancer and promotes tumor growth, metastasis, and chromosomal instability. We demonstrate that MYC, miR-17-5p, and miR-20a are up-regulated by CCAT2 through TCF7L2-mediated transcriptional regulation. We further identify the physical interaction between CCAT2 and TCF7L2 resulting in an enhancement of WNT signaling activity. We show that CCAT2 is itself a WNT downstream target, which suggests the existence of a feedback loop. Finally, we demonstrate that the SNP status affects CCAT2 expression and the risk allele G produces more CCAT2 transcript. Our results support a new mechanism of MYC and WNT regulation by the novel lncRNA CCAT2 in colorectal cancer pathogenesis, and provide an alternative explanation of the SNP-conferred cancer risk.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.080
GPT teacher head0.385
Teacher spread0.305 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations611
Published2013
Admission routes1
Has abstractyes

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