Dual Delivery of Placental Growth Factor and Vascular Endothelial Growth Factor from Poly(Hydroxyethyl Methacrylate-Co-Methyl Methacrylate) Microcapsules Containing Doubly Transfected Luciferase-Expressing L929 Cells
Why this work is in the frame
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Bibliographic record
Abstract
Placental growth factor (PlGF) combined with vascular endothelial growth factor (VEGF(164)) did not result in greater luciferase expression of a microencapsulated genetically transfected mouse fibroblast cell line (L929 cells) implanted subcutaneously in nominally syngeneic C3H/HeJ mice than in a control without growth factors. The intent had been to increase the maturity status of VEGF-generated vessels in the implant site by co-delivery of PlGF and thereby to effect an improvement in the persistence of luciferase expression, a marker of encapsulated cell viability. L929 cells were doubly transfected with luciferase and PlGF or luciferase and VEGF. Two hundred microcapsules containing a 1:1 mixture of the two transfected cells were implanted minimally invasively in Matrigel on one side of the mouse; the other side contained 200 microcapsules containing cells expressing luciferase only. Luciferase expression, reflective of encapsulated cell number, peaked at day 21 in both groups at about three times the value at day 1. In contrast with the immature vessels produced with VEGF alone (as reported earlier), the vessels produced here were more mature at day 14, and there were more such vessels than in the control group, although by day 21, there was a mixture of mature and immature vessels with PlGF, consistent with the premise that the maturity status of new vessels is PlGF dose dependent. Furthermore, anti-L929 antibodies (according to flow cytometry) and CD3-positive cells (according to histology) were detected in mice receiving unencapsulated cells, suggesting that there may be minor MHC (major histocompatibility complex) alloantigens and an adaptive immune response in this animal model. Thus successful modulation of the host response to microencapsulated cells may require enhanced vascularization and manipulation of the immune response, even with nominally syngeneic cells.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it