In vitro Translation of mRNA from Rat Peritoneal and Intestinal Mucosal Mast Cells
Bibliographic record
Abstract
We isolated RNA from in vivo-derived rat peritoneal (PMC) and intestinal mucosal mast cells (IMMC) and assessed the in vitro translation products of these two mast cell types using rabbit reticulocyte lysate and wheat germ systems. The translated polypeptides were analyzed by one- and two-dimensional gel electrophoresis. Fluorographic analyses established that polypeptides with a spectrum of molecular size and pI were produced by both PMC and IMMC. Immunoprecipitation studies with anti-rat mast cell protease (RMCP) I antibodies detected a band at 31 kD from products of PMC RNA assessed by one-dimensional electrophoresis, whereas anti-RMCP II antibodies precipitated a band of 27 kD from products of IMMC RNA. This is the first report of translation products of IMMC and of the translated precursors of RMCP I and RMCP II.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".