Enzymes of malate metabolism in<i>Mesorhizobium ciceri</i>CC 1192
Bibliographic record
Abstract
Electrophoretic studies were performed on enzymes concerned with the oxidation of malate in free-living and bacteroid cells of Mesorhizobium ciceri CC 1192, which forms nitrogen-fixing symbioses with chickpea (Cicer arietinum L.) plants. Two malate dehydrogenases were detected in extracts from both types of cells in native polyacrylamide electrophoresis gels that were stained for enzyme activity. One band of malate dehydrogenase activity was stained only in the presence of NADP+, whereas the other band was revealed with NAD+ but not NADP+. Further evidence for the occurrence of separate NAD- and NADP-dependent malate dehydrogenases was obtained from preliminary enzyme kinetic studies with crude extracts from free-living M. ciceri CC 1192 cells. Activity staining of electrophoretic gels also indicated the presence of two malic enzymes in free-living and bacteroid cells of M. ciceri CC 1192. One malic enzyme was active with both NAD+ and NADP+, whereas the other was specific for NADP+. Possible roles of the multiple forms of malate dehydrogenase and malic enzyme in nitrogen-fixing symbioses are discussed.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".