Vegetative storage proteins in the tropical tree <i>Swietenia macrophylla</i>: seasonal fluctuation in relation to a fundamental role in the regulation of tree growth
Bibliographic record
Abstract
The protein-storing cells in Swietenia macrophylla King were investigated. They were found to be of the Populus type, i.e., ordinary parenchyma cells containing both vacuole protein inclusion and starch grains. Vegetative storage proteins with molecular masses of 18 and 21 kDa were separated by SDSPAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Immunoblotting with the 21-kDa protein antiserum showed that the 18- and 21-kDa proteins shared common epitopes. The 21-kDa protein and presumably the 18-kDa protein were demonstrated by immunogold labeling to be the main components of the vacuole protein inclusion of the protein-storing cells. At the late stage of an annual growth cycle, vegetative storage proteins were found in the branchlets, trunk, large roots, and small roots. They were stored in large amounts in the secondary phloem of these organs and also in the secondary xylem of the terminal branchlets and small roots. In a new growth cycle, the consumption of the previously accumulated vegetative storage proteins began in the terminal branchlets of the last growth cycle. The vegetative storage proteins in the branchlets were exhausted completely when the new shoot leaves matured, while the storage proteins in the trunk and large roots had no detectable changes in abundance. On the other hand, the tree started to accumulate the two proteins in the stem of the new shoots as early as 1 week after the new shoot leaves matured. These results suggested that the previously accumulated vegetative storage proteins were used for new shoot growth and cambial activity in preference to the newly assimilated nitrogen and that vegetative storage proteins existed in considerable amounts in the stems throughout an annual growth cycle. This seasonal fluctuating pattern of vegetative storage proteins in the whole tree may be an important mechanism by which the tree regulates its growth.Key words: vegetative storage proteins, nitrogen metabolism, Populus-type of protein-storing cells, tropical hardwoods, Swietenia macrophylla King.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".