Physiological influences in the development and function of the shoot apical meristem of microspore-derived embryos of<i>Brassica napus</i>‘Topas’
Bibliographic record
Abstract
The effect of auxins and abscisic acid (ABA) on shoot apical meristem development and function was analyzed in microspore-derived embryos of Brassica napus L. ‘Topas’. Embryos were treated with an auxin transport inhibitor, tri-iodobenzoic acid (TIBA) or exogenous indole-3-acetic acid (IAA) at various stages during their development. If embryos were treated at the preglobular or globular stages of embryogenesis, they developed one fused cotyledon indicating a continuation of radial symmetry. However, if treated later in development, embryos were comparable to the control and formed two separate cotyledons. To demonstrate that this was not a pharmacological effect, an auxin antagonist, 2-(p-chlorophenoxy)-2-methylpropionic acid, included with the TIBA treatment produced normal embryos with two separate cotyledons. Histological observations revealed that the shoot apical meristem of the embryos treated with TIBA or IAA at the preglobular and globular stages was altered. Preglobular and globular stage embryos that were treated with TIBA exhibited a dramatic decline in conversion frequency compared with their controls. Embryos that were treated later in development had conversion frequencies comparable to their controls, and their shoot apical meristems also were similar to controls. Application of ABA at the preglobular and globular stages maintained meristem integrity and improved embryo conversion. However, ABA could not reverse the TIBA effect.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".