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Record W2008949664 · doi:10.1055/s-0029-1211207

Clinical Significance of In Situ Hybridization

2009· review· en· W2008949664 on OpenAlexaff
L. Sylvia

Bibliographic record

VenueExperimental and Clinical Endocrinology & Diabetes · 2009
Typereview
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicVirus-based gene therapy research
Canadian institutionsSt. Michael's Hospital
Fundersnot available
KeywordsIn situ hybridizationIn situBiologyImmunohistochemistryEndocrine systemReceptorPathologyHormoneMessenger RNABiochemistryImmunologyChemistryMedicineGene

Abstract

fetched live from OpenAlex

In situ hybridization is a morphologic method of localizing specific DNA or RNA sequences at the individual cell level. The technique can be applied to frozen or fixed tissues and cytologic preparations; various types of probes can be utilized and the reaction can be visualized by autoradiography using isotopic markers or by colorimetric methods. In the field of endocrinology, in situ hybridization has seen numerous applications; the detection of viral infections, the characterization of tumors with markers of endocrine differentiation, the determination of hormone synthesis by normal cells and neoplasms. Ectopic hormone production and the production of other substances such as growth factors and cytokines have been established using this new methodology and it has been used to examine production of specific receptors by individual cells in many endocrine tissues. The data that have emerged allow structure-function correlations that have not previously been possible using molecular techniques that are applied to homogenized tissues or even using the most sophisticated morphologic methods such as immunohistochemistry and electron microscopy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Other design · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: Review
Teacher disagreement score0.987
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0020.001
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.057
GPT teacher head0.431
Teacher spread0.374 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designOther design
Domainnot available
GenreReview

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2009
Admission routes1
Has abstractyes

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