CHARACTERIZATION OF PHYTOCHELATIN-LIKE COMPLEXES FROM FLAX (LINUM USITATISSIMUM) SEED
Bibliographic record
Abstract
Proteins were extracted from dehulled and delipidated powder of flaxseed (cv. NorMan), and fractionated by DEAE-Sephacel onion exchange chromatography. Ultraviolet absorbance scans of ion exchange fractions eluting in buffers at pH 8.6 containing 0.45 M and 0.50M NaCl revealed A255 >A280. Furthermore, the A255 was reduced upon acidification and restored by adjusting to the original pH, confirming spectral characteristics typical of metal-thiolate complexes. Characteristic circular dichroism spectra and reversible aggregation-dissociation behavior under low and high salt conditions observed for these fractions are also typical of metal binding phytochelatins reported for other plants, while the presence of aromatic amino acids indicated by fluorescence spectra suggests some similarity to nonmetallothionein proteins. Further work is underway to investigate the prevalence of these and other complexes as well as the distribution of metals in different protein fractions of flaxseed, as a function of cultivar and geographical location.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".