Characterization of the<i>sleepy sperm</i>mutant in the fern<i>Ceratopteris richardii</i>: A new model for the study of axonemal function
Bibliographic record
Abstract
Structural and motility characteristics of the zzz1 "sleepy sperm" mutant of Ceratopteris richardii Brongn. are described using scanning electron, transmission electron, light, and fluorescence microscopy. Although the zzz1 phenotype segregates as the product of a single gene mutation, the expression of the mutation varies within a single haploid gametophyte. The majority of mutant sperm cells are slow to initiate motility and typically swim in a slow, spiraling pattern. However, motility phenotypes range from immotile to wild-type (normal). This variable phenotypic expression is associated with a wide range of defects in the microtubule systems, especially the flagellar axonemes and the spline, a structure that provides a structural backbone for the cell. Defects in the spline microtubule array are associated with atypical cell shape and organellar positioning. Axonemal aberrations include an absence of the central pair complex and clumped flagella. We hypothesize that the gene product encoded by the zzz1 locus is not required for the establishment of the cytoskeletal elements necessary for sperm motility but rather is needed for stability and (or) repair (recycling) of these structures. This interpretation is consistent with the variable expression of zzz1 sperm, which appears to be age dependent.Key words: axoneme, microtubule, motility mutant, sperm cell, ultrastructure.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".