Multiple Effects of Serotonin and Acetylcholine on Hyperpolarization-Activated Inward Current in Locomotor Activity-Related Neurons in Cfos-EGFP Mice
Bibliographic record
Abstract
Hyperpolarization-activated inward current (I(h)) has been shown to be involved in production of bursting during various forms of rhythmic activity. However, details of I(h) in spinal interneurons related to locomotion remain unknown. Using Cfos-EGFP transgenic mice (P6-P12) we are able to target the spinal interneurons activated by locomotion. Following a locomotor task, whole cell patch-clamp recordings were obtained from ventral EGFP+ neurons in spinal cord slices (T(13)-L(4), 200-250 microm). I(h) was found in 51% of EGFP+ neurons (n = 149) with almost even distribution in lamina VII (51%), VIII (47%), and X (55%). I(h) could be blocked by ZD7288 (10-20 microM) or cesium (1-1.5 mM) but was insensitive to barium (2-2.5 mM). I(h) activated at -80.1 +/- 9.2 mV with half-maximal activation -95.5 +/- 13.3 mV, activation rate 10.0 +/- 3.2 mV, time constant 745 +/- 501 ms, maximal conductance 1.0 +/- 0.7 nS, and reversal potential -34.3 +/- 3.6 mV. 5-HT (15-20 microM) and ACh (20-30 microM) produced variable effects on I(h). 5-HT increased I(h) in 43% of EGFP+ neurons (n = 37), decreased I(h) in 24%, and had no effect on I(h) in 33% of the neurons. ACh decreased I(h) in 67% of EGFP+ neurons (n = 18) with unchanged I(h) in 33% of the neurons. This study characterizes the I(h) in locomotor-related interneurons and is the first to demonstrate the variable effects of 5-HT and ACh on I(h) in rodent spinal interneurons. The finding of 5-HT and ACh-induced reduction of I(h) in EGFP+ neurons suggests a novel mechanism that the motor system could use to limit the participation of certain neurons in locomotion.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".