Time Lapse Confocal Microscopy Studies of Bacterial Adhesion to Self-Assembled Monolayers and Confirmation of a Novel Approach to the Thermodynamic Model
Bibliographic record
Abstract
In this study, we use thermodynamic theory to develop a novel model that allows for the quantitative determination of the Gibbs free energy of adhesion for the initial bacterial attachment process. This model eliminates the need to calculate interfacial free energies and instead relies on easily measurable contact angles to determine DeltaG(adh). We experimentally verify our model using real-time observation of the initial attachment of Pseudomonas putida to methyl- and hydroxyl-terminated self-assembled monolayers. We also test the applicability of our model to a variety of experimental conditions using data available in the literature. We show that the initial attachment process is governed by dispersion forces and is accurately predicted by our model. Also, we find that our model is simple to apply and accurate for a variety of experimental conditions.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".