Effects of Chloride Substitution in Isolated Mesenteric Blood Vessels from Dahl Normotensive and Hypertensive Rats
Bibliographic record
Abstract
The purpose of this investigation was to examine the effect of Cl-free medium, nitric oxide synthase inhibitor (N nitro-L-arginine methyl ester; L-NAME), and Cl channel antagonist (niflumic acid), on alpha1-adrenoceptor (cirazoline) mediated responses in the isolated mesenteric blood vessels from Dahl salt-resistant normotensive (SRN) and salt-sensitive hypertensive (SSH) rats on a 4% salt diet for 7 weeks. Cirazoline produced dose-dependent vasoconstriction in blood vessels of SRN and SSH rats. Replacement of extracellular Cl with propionate ions significantly inhibited (P < 0.05) cirazoline-mediated vasoconstriction in SRN but not in SSH rats. Perfusion with L-NAME (10 microM) augmented responses to cirazoline in SRN but not in SSH rats. In Cl-free medium, addition of L-NAME had a biphasic effect on cirazoline responses; potentiation of responses at the lower doses and attenuation at the highest dose. Niflumic acid (10 microM) significantly inhibited cirazoline responses with the inhibition being more pronounced in SRN than SSH rats. The resting Em of smooth muscle cells was -68.0 +/- 4.2 mV (mean +/- SD; n = 87) and -67.2 +/- 4.8 mV (n = 88), in SRN and SSH rats, respectively. Perfusion with Cl-free medium produced a significant depolarization that was larger in smooth muscle cells of SSH (-57.4 +/- 4.8 mV, n = 38) than SRN (-61.3 +/- 5.4 mV, n = 35) rats, while L-NAME depolarized the smooth muscle cells of SRN (-62.1 +/- 6.5 mV, n = 36) but not SSH (-67.5 +/- 4.2 mV, n = 34) rats. The data supports the view that Cl handling and Ca-dependent Cl channels seem to undergo modification as a consequence of salt-induced hypertension. It is also possible that the modified role of nitric oxide on membrane potential may have a direct bearing on the changes observed in Cl handling in blood vessels of SRN versus SSH rats.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".