Analysis of total DNA by minisatellite and simple-sequence repeat primers for the use of population studies in<i>Plasmopara halstedii</i>
Bibliographic record
Abstract
Total DNA of Plasmopara halstedii isolates from Germany was analysed for polymorphisms potentially useful for the differentiation of field isolates with respect to epidemiological studies or pathotype characterization. The isolation of the DNA started from mitotically formed zoosporangia, which is the only cellular structure of the biotrophic pathogen accessible independently from its host. The total DNA of the pathogen was used to perform DNA fingerprints with minisatellite and simple-sequence repeat primers. Polymorphisms were found that allowed differentiation on the level of single field isolates; however, they were not correlated with either physiological races or the geographic origin of the isolates. Using such differentiating primers, single spore strains of three pathogen isolates were also analysed with respect to genetic homogeneity. Minor variation was visible in the mitotically derived offspring, but the overall appearance of these patterns was mostly uniform with those of the respective parental isolate.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".