A novel method of measuring gas phase nitric oxide concentration in cell cultures
Bibliographic record
Abstract
This is the first report on the measurement of nitric oxide (NO) concentrations in the gas phase in a squamous cell culture. The technique may permit the assessment of NO output under conditions that aim to mimic facets of pathology in relatively inaccessible tissues. The primary aim of this study was to devise a method to detect the NO concentration produced by cell cultures in the gas phase of a culture flask. A secondary aim was to determine whether the effect of hypoxia or radiation on NO production in a human squamous cell carcinoma cell culture would be detectable with this technique. The NO concentration was measured off-line using a rapid-response chemiluminescent analyzer. The gas samples were aspirated from cell culture flasks (i) under normal culture conditions, (ii) under conditions of hypoxia and (iii) following radiation of human head and neck squamous cell carcinoma cell line cultures. Elevated levels of the gas phase NO concentration were consistently obtained from the cell culture flasks using this experimental set-up. Hypoxia and radiation diminished NO production.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".