Purification and characterization of<i>Enterobacter sakazakii</i>enterotoxin
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Enterobacter sakazakii has recently been recognized as an often fatal neonatal pathogen that rarely infects adults. Although not much is known about factors involved in its pathogenicity, the organism has been reported to produce enterotoxin. Currently, no information is available in the literature about the production and characterization of the enterotoxin. This report is the first attempt regarding purification and biochemical characterization of the enterotoxin produced from E. sakazakii. The toxin was purified by ammonium sulfate precipitation, followed by DEAE cellulose ion exchange and desalting by Sephadex G-100. The 66 kDa toxin was most active at pH 6 and was stable at 90 degrees C for 30 min. This stability combined with the potent activity of the toxin (LD50 = 56 pg) emphasizes the potential risk to neonates fed infant milk formula contaminated with E. sakazakii. Further detailed molecular biological studies on the toxin are warranted in view of its stability and activity.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it