EFFECT OF BAOHUOSIDE-1 (B1), A NOVEL IMMUNOSUPPRESSANT, ON CELL ACTIVATION, CELL CYCLE, APOPTOSIS, AND EXPRESSION OF IL-2 AND IL-2R AND PROLONGATION OF HEART ALLOGRAFT SURVIVAL IN RATS
Bibliographic record
Abstract
P1110 Background: Immunosuppressive effects of Baohuoside-1 (B1), a novel flavonoid isolated from Epimedium, were evaluated in vitro and in vivo in this study. Methods: Proliferation assay was used for determine the antiproliferative prosperities. Flow cytometry analysis was applied to detect the changes of phenotypes on activated cells. Analysis of Western blotting was employed to observe the expression of cyclins and cdks proteins. Apoptosis induction was analysed by annexin V/PI staining. Levels of IL-2 and IL-2R proteins were evaluated by ELISA and FACS. A model of rat heart transplantation was used to further determine the immunosuppression of B1 in vivo. Results: The major findings are as follows: (1) B1 has immunosuppressive effect on MLC with IC50 of a low μM concentration. (2) B1 effectively inhibits the proliferation of human T and B cells triggered by IL-2 or mitogenic antigen stimulation in a dose and time dependent fashion. (3) B1 resulted in G1−S phase cell arrest. (4) B1 down-regulated the expression of cyclin A and p33cdk2 proteins on IL-2 signalling, but had only marginal effect on expression of cyclin D protein. (5) B1 could induce apoptosis and caspase-3 activation in human T lymphocytes. (6) B1 declined partially the level of secretion of IL-2 protein and expression of IL-2R. (7) The synergistic immunosuppressive effect between B1 and FK506 was found in the CD69 expression stimulated by PMA/CD28 and PMA/iono, but not by CD3/CD28. (8) Furthermore, synergistic effect of B1 combined with FK506 was also observed by prolonging MST in rat heart transplant model in vivo. Conclusions: The immunosuppression of B1 on cell activation takes place in G1−S transition and is associated with expression of IL-2 and IL-2R, apoptosis induction and with expression of cyclin A and p33cdk2 proteins. B1 has selectively immunosuppressive effects on T and B cell activation in vitro and in vivo.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".