Calorimetric characterization of critical targets for killing and acquired thermotolerance in yeast
Bibliographic record
Abstract
We characterized thermal behaviours of cellular components by differential scanning calorimetry (DSC) in order to investigate how Saccharomyces cerevisiae cells acquire thermotolerance after heat shock or in stationary phase. Whole-cell DSC profiles consisted of at least five endothermic components over the range 45-75 degrees C for exponentially growing, heat-shocked and stationary-phase cells. In these profiles, we attempted to localize the endothermic profiles due to denaturation of the two critical targets which were predicted by using the Arrhenius parameters of hyperthermic killing of the cells (Obuchi et al., 1998). This prediction indicated that (a) the heat shock stabilized one family of targets and destabilized the other, while (b) arrest in stationary phase stabilized both targets. Therefore, the heat-shock response does not stabilize all cellular components, and arrest in stationary phase appears to stabilize cellular components in a different manner from the heat-shock response. It was not possible unambiguously to resolve the profiles of the critical targets in the DSC scans of whole cells. Components I (T(m)=49.7 degrees C) and II (T(m)=56.1 degrees C) may both include denaturations of critical targets 1 (T(m)=55.4 degrees C) and 2 (T(m)=53.0 degrees C) in exponential cells. Components I and II were both stabilized (T(m)=53.5 and 57.2 degrees C, respectively) in heat-shocked cells. Sub-cellular fractions suspended in 1.2 M trehalose solution, which mimics the cytosol in tolerant cells, were more stable than those in 0.6 M KCl, which mimics the cytosol in sensitive cells. The microsomal fractions in KCl and trehalose had endothermic profiles in similar temperature ranges to those predicted for sensitive and tolerant cells, respectively. This agreement suggests that the microsomal fraction may contain critical targets, and that trehalose accumulation in the heat-shocked and in the stationary phase yeast cells is a stabilizer of cellular components.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".