364 DISTRIBUTION OF OVULATION AND SUBSEQUENT EMBRYO PRODUCTION USING LUTROPIN AND ESTRADIOL-17ß FOR TIMED AI OF SUPERSTIMULATED BEEF FEMALES
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Bibliographic record
Abstract
This experiment was designed to compare timing of ovulation and embryo production between traditional superstimulatory and AI methods and the use of additional treatments of Estradiol-17ß and pLH (Lutropin; Bioniche Animal Health, Belleville, Ontario, Canada) to facilitate timed AI in beef cows. A total of 31 mature cross-bred beef cows were randomly assigned three treatments. On Day 0, experimental cows were selected upon transrectal ultrasound evaluation for corpus leutum (>10 mm) prior to CIDR insertion plus 2.5 mg estradiol-17ß and 50 mg Progesterone i.m. Superstimulatory treatments with Folltropin-V (Bioniche Animal Health) began on Day 4 for 3.5 days (236 mg NIH-FSH-P10) in decreasing dosages (7 injections at 0700 and 1900). All cows received prostaglandin F (PGF) treatments of 625 mcg D-cloprostenol i.m. (Estrumate; Schering-Plough, Union, NJ, USA) in both AM and PM on Day 6, and CIDRs were removed in AM of Day 7, followed by the final injection of Folltropin. In addition, Heat Watch patches (Heat Watch; Cow Chips, Denver, CO, USA) were applied to optimize heat detection. Donors selected as control treatment were AI at 12 and 24 h post-onset of estrus (first mount) with frozen semen from the same bull. Donors selected on the Estradiol-17ß treatment received 1 mg i.m. 12 h after CIDR removal and timed AI at 24 and 36 h. Donors selected for Lutropin treatment received 12.5 mg (5cc i.m.) 24 h after CIDR removal and were timed AI at 12 and 24 h. All AI procedures used 2 units from the same bull. All cows were examined by ultrasonography every 8 h beginning 24 h post-CIDR removal and ending at 60 h to determine the time and distribution of ovulation. Ovulation was determined by the disappearance of follicles (>12 mm) from the prior examination. All AI and ultrasound procedures were performed by the same technician. There were no differences detected between the three treatments as evaluated by all the variables we studied (Table 1). However, donors treated with Lutropin tended to produced more viable embryos per collection and had a tighter degree of distribution of ovulations. Donors treated with Estradiol-17ß tended to have fewer viable embryos. More research is needed to determine if donors can be timed AI without regard to estrus. Table 1. Mean onset of estrus, distribution of ovulation, and embryo production in superstimulated beef donors treated with Estradiol-17ß, and Lutropin
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it