Generation of a Monoclonal Antibody that Blocks Epithelial Binding of Unopsonized Particles
Bibliographic record
Abstract
Alveolar macrophages (AMs) and epithelial cells (ECs) are the first cells in the lung to encounter inhaled environmental particles. The initial interaction between AMs and particles is mediated by specific scavenger receptors, but the nature of the structure(s) on ECs that also bind particles has not been well-described. To characterize the nature of the EC particle receptor, we screened a panel of mouse anti-human EC hybridomas for functional blockade of EC particle binding. This strategy identified a monoclonal antibody (MAb) (EPR1) that blocks binding of titanium dioxide (TiO(2)) particles to the EC line which served as the immunogen (A549), as well as to other EC lines (Beas 2-B, HTB54, HeLa, and MDA-MB-435S). EPR1 demonstrated specific labeling of ECs using immunohistology techniques and its expression could be quantitated by flow cytometry of permeabilized ECs in suspension. MAbs such as EPR1 may prove useful in further analysis of receptors for inhaled particles on lung epithelial cells.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".