Early plasma membrane events occurring in ultraviolet-B-induced apoptosis
Bibliographic record
Abstract
Whereas nonsolar ultraviolet C radiation primarily affects nuclei (i.e., where it is absorbed by nucleic acids) of eukaryotic cells, ultraviolet radiation of long (320-380 nm) wavelengths (ultraviolet A) and intermediate (290-320 nm) wavelengths (ultraviolet B) primarily affects lipid membranes. We have previously demonstrated that ultraviolet B irradiation alters the surface architecture of human B cells and impairs expression of an erythroid growth factor on their surface and on extracellular vesicles. Here, we examined the effects of ultraviolet B irradiation on the capacity of Chinese hamster ovary cells to undergo the process of exfoliation, and on the capacity of Chinese hamster ovary cells transfected with flt3/flk2 cDNA to express the cytokine flt3/flk2. Our results indicate that the rate of release of shed vesicles from untransfected Chinese hamster ovary cells is decreased after one to two h, at a time when there is electron microscopic evidence for retention of vesicles at the cell surface. These changes at the cell surface precede all other apparent morphological changes (including DNA condensation in the nucleus, swelling of the mitochondria and appearance of apoptotic bodies). Furthermore, plasma membranes and shed extracellular vesicles from ultraviolet B irradiated Chinese hamster ovary cells that have been transfected with flt3/flk2 cDNA fail to express the protein.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".